Stem Cell Treatment for Rejuvenation

Related Articles Synergy between Choroid Plexus Epithelial Cells-Conditioned Medium and Knockout Serum Replacement Converts Human Adipose-Derived Stem Cells to Dopamine-secreting Neurons. Rejuvenation Res. 2017 Mar 02;: Authors: Boroujeni ME, Gardaneh M, Shahriari MH, Aliaghaei A, Hasani S Abstract Human adipose-derived stem cells (hADSCs) have great capacity to differentiate into mesodermal origins as well as non-mesodermal lineages including neural cells. This valuable feature paves the way for the therapeutic application of hADSCs for neurodegenerative maladies such as Parkinson's disease (PD). We tested the capacity of Choroid Plexus epithelial cells-conditioned medium (CPEC-CM) alone or cocktailed with knock-out serum (KS) to induce dopaminergic differentiation of hADSCs. To this end, hADSCs from lipoaspirate were phenotypically characterized and shown to maintain mesodermal multipotency so selected media easily differentiated them into osteoblasts, chondrocytes and adipocytes. To begin inducing hADSCs neuronal differentiation, we isolated CPECs from rat brain and expanded in culture in order to obtain CPEC-CM. We then treated hADSCs with optimized quantities of collected CPEC-CM, knock-out serum (KS) or both. The ADSCs treated with either CPEC-CM or CPEC-CM and KS displayed morphological changes typical of neuron-like phenotypes. As revealed by RT-PCR, qPCR and immuno-staining analyses, hADSCs co-treated with CPEC-CM and KS expressed significantly higher levels of neuronal and dopaminergic markers in comparison with single-treated groups. Moreover, the hADSCs began expressing dopamine-biosynthesizing enzymes mainly after co-treatment with CPEC-CM and KS. Consequently, only co-treated hADSCs were capable of synthesizing and releasing dopamine detectable by HPLC. Finally, hADSCs growing in ordinary medium were found positive for astrocytic marker GFAP, but stopped GFAP expression upon either single or co-treatments. These combined results suggest CPEC-CM and KS can synergize to remarkably augment dopaminergic induction of hADSCs, an effect that has implications for cell replacement therapy for PD and related disorders. PMID: 28437187 [PubMed - as supplied by publisher]